Almost every cell in the body of living organisms contains DNA – the basis for life. From DNA, proteins are constantly being made that carry out cellular functions. Interestingly, only a small percentage of the genome codes for proteins (coding regions). What then, is the rest of the DNA (non-coding regions) for? Scientists once thought it was simply “junk,” but we now know that at least a portion of it serves a role in telling coding regions when and how fast to make proteins. This is accomplished via interactions of proteins with specific and relatively short DNA sequences in the non-coding regions, known as regulatory regions. Using a method called chromatin immunoprecipitation sequencing (ChIP-seq), these regulatory regions can be mapped or “annotated” across the entire genome so that their specific locations can be visualized. This allows researchers to determine if mutations in the vast expanse of non-coding DNA are likely to have any effect on the coding regions and thus on production of proteins important for cell function. ChIP-seq has been performed for humans and a variety of other species, and these species therefore have genomes that are well annotated for regulatory regions. For the cat genome, no such annotation exists. This study proposes to correct this deficit by performing ChIP-seq on eight different organs collected from cats after euthanasia for unrelated health reasons at the Texas A&M Veterinary Medical Teaching Hospital. This data would be made publicly available to aid in interpretation of feline genomic data.