Ringworm is a condition affecting cats that is caused by a fungal infection. Humans and pets can acquire ringworm from infected cats. Shelter cats are at high risk and should be screened before being introduced to other pets. The current method of testing, fungal culture, may take weeks to provide a definitive answer. This study involves the development of molecular techniques to provide rapid diagnosis so cats can be effectively treated, thus reducing the threat of owners and other pets contracting ringworm.