Diabetes mellitus is one of the most common endocrine diseases of cats but relatively little is known about the factors responsible for its development. Glucose (blood “sugar”) is abnormally elevated in diabetic cats. It is also well known that even normal cats can develop elevated blood glucose under some circumstances, such as a stressful event. In contrast to cats, “stress” hyperglycemia, as the elevation in blood glucose is called, is unusual in dogs. The propensity of cats to develop stress hyperglycemia along with other peculiarities in the regulation of blood glucose suggests the possibility that cats may possess unique physiologic mechanisms for glucose control. The purpose of this study is to develop a novel method to study glucose metabolism in cats using polymerase chain reaction (PCR) technology. The results of this study will shed light on the normal glucose metabolism of cats and provide the foundation for additional studies into the role of abnormal glucose metabolism in the development of feline diabetes.