Precision/Genomic Medicine using genetic information of an individual to determine the cause of a specific health problem, which is the new, fancy, terminology for the research efforts the Lyons laboratory has pursued for the past 20 years. In addition, Precision/Genomic Medicine can also help determine genes involved in a biological pathway of a health condition and those genes can then be targeted by specific drugs and therapies, leading to not only an identification of a cause for a disease but also a potential treatment or cure. The 99 Lives Cat Genome Sequencing Project is a consortium of investigators pursuing Precision Medicine in cats. The Lyons laboratory at the University of Missouri leads the project by collating all the cat genomes from diverse investigators and veterinarians and conducting the genetic analyses of these sequences to help find the DNA variants causing heritable diseases and traits in cats. These analyses cannot be performed by many of the veterinarians and investigators interested in cat health, therefore, they benefit from the consortium. Workflows and pipelines for processing the genome sequences have been developed by previous funding. However, a new reference for the cat genome has been produced, thus, all the cat genomes in the consortium, which is >400 genome sequences, need to be re-evaluated with the new cat reference. The data from this re-evaluation with then be provided to the cat research community. This new dataset will be key to the continued discovery of DNA variants causing cat health problems and traits.