EC26S-052: Development of a differential antigen-based rapid screening test for use in FIP-suspect cats

The goal of our study is to develop a rapid diagnostic test for FIP, utilizing a set of two monoclonal antibodies developed at Cornell University. Each of these antibodies specifically recognize the spike protein of either FCoV-1 or FCoV-2. To test the antibodies, we will use samples from cats, some with confirmed FIP, some suspected FIP and healthy cats as controls. We will test the antibodies in abdominal fluid, eye swabs, blood, and also in tissues sent to our lab from cats that have passed away. We will use immunological assays like ELISA and protein gel analysis to evaluate if our antibodies can differentiate FCoV-1 and FCoV-2 infection in cats. Our antibodies will also be compared to a commercial antibody against FCoV, and the immunological assay results will be compared to genetic testing like PCR and viral sequencing. We will also sequence the antibodies and use this result to mass produce them in our lab and to determine exactly which part of the spike protein they can recognize. This will ensure that the antibodies work against the various strains of FCoV-1 and FCoV-2 that circulate around the world. In the end, we expect this work to confirm that these antibodies can be utilized to develop a fast and reliable diagnostic test for FIP. (Bria Fund)