Flow cytometry to assess oxidative stress in cats

Winn Funded Research

Webb, C., C. Bedwell, et al. (2006). “Use of flow cytometry and monochlorobimane to quantitate intracellular glutathione concentrations in feline leukocytes.” Vet Immunol Immunopathol 112(3-4): 129-40.

Oxidative stress and abnormal glutathione metabolism is thought to play an important role in various diseases of cats. However, current assays for the reduced form of glutathione (GSH) are time-consuming and semi-quantitative and do not allow assessment of GSH concentrations in individual cell populations. Therefore, we developed a flow cytometric assay for rapid determination of intracellular GSH concentrations in feline blood leukocytes.

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