Nonregenerative cytopenias such as nonregenerative anemia, neutropenia, and thrombocytopenia are a common finding in cats infected with feline leukemia virus (FeLV). The objective of this study was to assess the role of latent FeLV infection in bone marrow in cats with nonregenerative cytopenias that had a negative FeLV antigen blood test. Thirty-seven cats were included in the patient group meeting inclusion criteria of a nonregenerative cytopenia of unknown origin and a negative FeLV antigen test result. The group’s results were compared to two control groups that also totaled 37 cats. Whole blood and bone marrow samples were tested for FeLV using two different PCR assays. Results indicated that PCR from bone marrow could be considered a more sensitive method to detect FeLV latency than PCR of whole blood. Two of the 37 cats (5.4%) in the patient groups were positive on bone marrow PCR results and were considered infected with FeLV. The latent FeLV infection in those two cats was the possible cause of the observed nonregenerative cytopenia. [VT]